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Development and Optimization of a Surface Sterilization Protocol for the Tissue Culture of Pleurotus Tuber-Regium (Fr) Sing. And Auricularia Auricula-Judae

Authors

  • Okereke, O.E.
  • Akanya, H.O.
  • Ogbadu, G.H
  • Egwim, E.C.
  • Etim
  • Akande, S.A.

Abstract

This study was carried out to develop and optimize a surface sterilization protocol for the sterilization of explants in the tissue culture of the edible mushrooms Pleurotus tuber-regium (Fr) Sing. and Auricularia auricula-judae. The explants were exposed to two sterilization agents; 70% ethanol (EtOH) and JIK® a locally produced bleaching solution containing 3.5% (w/v) sodium hypochlorite at concentrations of 2.5% (v/v) and 5.0% (v/v) with varying time intervals of one, two, five, ten and fifteen minutes. The results showed that treatment of explants with 5.0% (v/v) sodium hypochlorite solution for ten minutes preceded by 70% ethanol pre-treatment for two minutes gave the least explants contamination (0%) and highest explants survival (100%) for Pleurotus tuber-regium, while treatment of explants with 70% ethanol for one minute followed by 5.0% (v/v) sodium hypochlorite solution for five minutes, gave the highest survival rate (100%) and least explants contamination (0%) for Auricularia auricula-judae.

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Published

25-05-2022 — Updated on 15-12-2017

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How to Cite

Okereke, O., Akanya, H., Ogbadu, G., Egwim, E., Etim, V., & Akande, S. (2017). Development and Optimization of a Surface Sterilization Protocol for the Tissue Culture of Pleurotus Tuber-Regium (Fr) Sing. And Auricularia Auricula-Judae. British Journal of Multidisciplinary and Advanced Studies, 1(1), 1–9. Retrieved from https://bjmas.org/index.php/bjmas/article/view/1 (Original work published May 25, 2022)